Tuberculosis-specific T cells exist in the peripheral blood of patients infected with mycobacterium tuberculosis. After being stimulated by tuberculosis-specific proteins again, tuberculosis-specific T cells will rapidly activate and proliferate, secreting tuberculosis-related cytokines.
The Kit co-cultures human peripheral blood mononuclear cells and fusion proteins on cell culture plates. Tuberculosis-specific cells can secrete y-interferon (IFN-y) and interleukin-2 (L-2) factors due to memory reactions, and then adopt double antibody sandwich enzyme-linked immunoassay to detect the concentration of IFN-y and L-2 in the cultural supernatant, thus determining whether mycobacterium tuberculosis has a specific cellular immunoreaction. This method is not affected by BCG vaccine and other non-tuberculous mycobacteria, applicable to the detection of active tuberculosis.